Curated OER
Bacterial Transformation: Laboratory Experiment
Young scholars participate in a group lab in which they complete the process of bacterial transformation. If lab procedures are followed correctly, Students see their results in a few days...bacteria glowing green.
Curated OER
Bacterial Transformation - Biology Teaching Thesis
Students explain the following processes: bacteria transformation, genetic recombination, and antibacterial breakdown of bacteria. They follow lab protocol and use the scientific method: to make predications, and to explain an unexpected...
Curated OER
Bacterial Transformation
Students discuss a bacterial experiment they previously performed and complete a pre-assessment. They participate in a lecture discussion given by the teacher through PowerPoint and then complete a cartoon worksheet and post-assessment.
Curated OER
CELL TRANSFORMATION IN TOBACCO LEAF DISKS
Students work with whole plant material and are not required to measure small quantities, yet they can see evidence of transformed plant cells (plant cells that have genes from bacterial plasmids). This is a laboratory suitable for...
Towson University
Transformation Lab
Transform your class' understanding of genes and antibiotic resistance with the Transformation Lab. Junior geneticists create and observe their own resistant strains of E. coli through research, discussion, and experimentation. The...
Curated OER
Producing a Strain of E. coli that Glows in the Dark
Students observe the experimental process called bacterial transformation and demonstrate phenotype changes in bacteria that have been transformed with an antibiotic-resistance gene and a metabolic marker. They create a luminescent...
Curated OER
Transformation With A Firefly Gene
Students understand the process of bacterial transformation through experimentation. They describe the function of two important genes of the pBestLuc plasmid. They explain how to induce competence in E. coli cells.
Curated OER
Bioterrorism: Development of a "Superbug"
High schoolers compare bacteria and viruses and their roles in biotechnology and bioterrorism. They outline fundamental steps of bacterial transformation and the possible selection processes to identify transformants. They discuss...
Curated OER
Oswald Avery & The Search for the Transforming Factor
Five questions about the work of biochemist, Oswald Avery, are presented on this worksheet. You will need to find a reading passage, perhaps on the Internet, about his discovery, but it would be a worthwhile search. The assignment brings...
Curated OER
Bacterial Transformation
Students explore DNA recombination. In this biology lesson plan, students observe the growth of microscopic species. They predict the phenotype of the transformed colonies.
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Genetic Engineering/Biotechnology
Students create a paper model of a plasmid and insert a DNA molecule that contains a specific gene into the plasmid. They complete an actual transformation involving E. coli bacterium and relate the two activities to one another.
Curated OER
Cell Transformation in Tobacco Leaf Disks
Students review the basic principles of plant cell structure, tissue culture, sterile technique, and cell transformation. Next students complete a plant transformation experiment using whole plant material in order to view evidence of...
Curated OER
Simulation of Gene Splicing
Students use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that genetic...
Curated OER
Bacterial Transformation Kit
Students participate in an experiment in which they practice moving genes from one organism to another. They examine the results of a recombination of jellyfish. They identify different types of bacteria as well.
Nuffield Foundation
Following Gene Transfer by Conjugation in Bacteria
After the lab, you'll be able to solve this analogy: Natural selection is to vertical transfer of genes as ___ is to the horizontal transfer of genes. Young biologists conduct an experiment on E. coli bacteria to explore the process of...
Curated OER
What Color is Your Colony?
Students conduct an experiment to change the color of E. coli bacteria using genetic engineering. They work in small groups to add a plasmid containing a gene for red pigment to the bacteria and culture the bacteria to see if it ...
Curated OER
Biotechnology
The concepts here cover the gamut of biotechnological engineering. Information is given about plasmids and genetic manipulation. After reviewing genetic engineering, this slide show would be useful to recap some of the main procedures.
Curated OER
Phenotypic and Genotypic Changes in Bacteria
Students investigate a rapid method to render the bacterium Escherichia coli "competent" to uptake plasmid DNA. They demonstrate the phenotypic changes associated with transformation. They perform a cell resuspension from the...
Curated OER
PHENOTYPIC AND GENOTYPIC CHANGES IN BACTERIA
Students use a rapid method to render the bacterium Escherichia coli "competent" in order to uptake plasmid DNA. They also have growth on the plain LB plates by the untransformed cells (which serve as controls). Students perform a cell...
Curated OER
Simulation of Gene Splicing
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
Curated OER
Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
Curated OER
DNA's Discovery and Structure
With a professional appearance, this presentation introduces advanced biology learners to the history and structure of the DNA molecule. The details included in this resource are definitely more in-depth than what you would expose a...
Curated OER
Enzyme and Southern Blotting
Young scholars study southern blotting and its uses. They research on restriction enzymes and their uses, especially in relation to recombinant DNA. In addition, they write a summary and an interpretation of the gel.
Curated OER
DNA: The Genetic Material
The various experiments and processes that were completed in the 20th century to further our understanding of DNA are summarized here. There are wonderful slides to popularize the scientists who dedicated their professional life to...