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Curated OER
DNA Race Track
Twelfth graders simulate a DNA fragment running through a gel electrophoresis in a class activity. Students work in teams as a DNA sample with each person on the team representing a fragment that moves through the gel at a specific speed.
Curated OER
DNA Fingerprinting for High School Biology
Students work with tools and techniques used in genetic engineering. They dissect a sample into fragments of DNA using restriction enzymes, sometimes called "molecular scissors". They sort these fragments, measure and analyze them according
Curated OER
Characterization of the Bioluminescence (Lux+) Gene E. coli
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
Curated OER
Gel Electrophoresis
High schoolers conduct a variety of experiments to explore gel electrophoresis. In this biology lesson, explain how this process separate DNA and RNA. They discuss the practical applications of this method.
Towson University
Case of the Crown Jewels
Can your biology class crack the Case of the Crown Jewels? Junior forensics experts try their hands at DNA restriction analysis in an exciting lab activity. The lesson introduces the concept of restriction analysis, teaches pipetting and...
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COMPARISON OF FOUR-, SIX-, AND EIGHT-bp CUTS IN CALF THYMUS DNA
Students use this laboratory exercise, restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. Assuming that the four component nucleotides (A, C, T, G) are distributed randomly within a DNA molecule, then any...
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Comparison of Four-, Six-, And Eight-bp Cuts in Calf Thymus Dna
Students investigate the use of restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. They utilize restrictive endonucleases to cut Thymus DNA and compare results by viewing the DNA fragments on Agarose Gel...
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The Cloning of Plasmid and Spinach DNA
Students will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in...
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DNA Fingerprinting
Ninth graders investigate what restriction enzymes do, and explain how a DNA sample can link a suspect to a crime.
Curated OER
Cloning of Plasmid and Spinach DNA
Students ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and outcome of...
Curated OER
From Restriction Maps to Cladograms
High schoolers develop a matrix to determine the number of differences between each set of primate species. They determine closely related organisms then complete the cladogram and answer a set of questions.
Curated OER
Gel Electrophoresis Simulation
Learners identify the steps in a gel electrophoresis experiment and describe the role of enzymes. They examine the relationship between fragment size and migration rate in a gel. They view DNA fragments as well.
Curated OER
Simulation of Gene Splicing
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
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Wolves: DNA Pawprinting
Students explore and research the most widely distributed terrestrial carnivore, the wolf. They critique the true mystery of this animal and then form an opinion about this creature. The restriction-site analysis of mitochondrial DNA is...
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How to Conquer a Genetic Disease
Students study the details of DNA restriction analysis and how RFLP's are used to locate genes on a map of DNA.
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Genetics and Molecular Biology
Young scholars create DNA models using colored construction papers. In this biology lesson, students identify enzyme restriction sites. They model how DNA plasmids replicate when taken up by a bacterial cell.
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Wolf Pack in a Bottle
Students participate in an activity of a mock electrophoresis using paper chromatography to study DNA and genetic restriction mapping.
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The Great Cafeteria Caper
Students extract DNA from their own hair roots. They run an electrophoresis gel to examine patterns of their DNA along with standard DNA. The experiment is based on a crime scene scenario.
Virginia Department of Education
Biotechnological Issues and Bioethics
Culminate a bioethics unit with the implementation of a lesson that incorporates the Socratic method to encourage class feedback and participation. Pupils participate in a discussion on bioethics and morality, complete a...
Curated OER
Recombinant DNA - Electrophoresis Demonstration and Problem
Suitable for an AP Biology class, the lab found here has teen bioengineers working with plasmids to clone DNA. If you have not done a similar lab, the instructions may be rather difficult to follow. Both the teacher and learner...
Curated OER
Macro-DNA: Doing DNA Fingerprinting
Students explore gel electrophoresis using paper models. They model DNA sequences and shade the boxes on their Gel Electrophoresis Sheet which represent the individual fragments.
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Cutt-Off Genes
Learners explore how gene sequence analysis can be used to examine phylogenetic similarities of different organisms. Students work in groups to simulate a gel electrophoresis separation of fragments using poster board to create their gel.
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Cut-Off Genes
High schoolers explain the concept of gene sequence analysis. In this gene lesson, students draw inferences about phylogenetic similarities of different organisms.
Curated OER
Trichinella Forensics
Students engage in DNA manipulation using forensics techniques. Students investigate topics involved in forensics studies such as cloning, electrophoresis, gene mapping, replication, and transcription.