Teach Engineering
Chromatography Lab
Groups use alcohol and chromatography paper to separate the color components of black ink. The purpose of the activity is to allow the class to become aware that mixtures exist in hidden places.
Oregon State
Using Paper Chromatography
Through the analysis of paper chromatography to separate ink from the pen found at the crime scene, learners analyze the different stains and through calculations determine the thief.
Curated OER
#13 Chromatography is a Gas: An Inquiry-Based Introduction to Gas Chromatography
Advanced chemistry scholars read about gas chromatography as a means of separating mixtures into their components. Then they try the technique on fingernail polish remover. Detailed instructions are provided via a lab sheet for your...
Pingry School
Liquid Chromatography
A hands-on experiment applies chromatography to separate both the colors and components of grape-flavored Kool-Aid. Performing two separate activities, learners first use chromatography to divide the colors. The second activity results...
Curated OER
Chromatography Lab
Students discover the components of primary and secondary colors. In this physical science lesson, students create a set up in which water will separate a color into the component colors along a piece of filter paper. Students will then...
Olomana School
Mixtures and Solutions: Paper Chromatography Experiment
Why does some ink bleed through paper, and other ink doesn't? Practice some paper chromatography to separate the colors from a pen with an interactive experiment for middle and high schoolers. Learners use a variety of solutions to track...
Sunlight Cal-Tech
Chromatography of Plant Pigments
Through a hands-on activity, an acetone-spinach solution is pre-made and learners use this solution to separate the pigments found in spinach using chromatography. The comprehensive resource includes an analysis and conclusion questions.
LABScI
The Separation Lab: Candy Colors
There's no better motivator than candy! An engaging activity has pupils explore methods for separating mixtures. A two-part lab first explores methods of separation based on size and magnetism. The second activity introduces learners to...
Curated OER
Lipstick Chromatography/Ink Chromatography
Junior crime scene investigators are instructed in two different chromatography exercises. For female suspects, they separate and compare lipstick pigments. For male suspects, they separate and compare ink samples. A data sheet is...
Curated OER
Chromatography
Advanced techniques for the lab use of chromatography are detailed here and your students will learn the specifics of separating molecules using a staionary and mobile medium. Diagrams of the equipment used and some facts about how...
Curated OER
Recognition and Classification of Mixtures
Immerse your chemistry class in solutions! They melt and compare the mixtures that make up margarine, separate black ink into its component colors, distill ocean saltwater, and practice chromatography with plant pigments. There are eight...
Curated OER
Chromatography
Learners use chromatography to separate substances in Kool-Aid, Crayola markers and leaves. In this chromatography lesson plan, students make chromatograms to separate substances in a mixture through absorption. Learners find the Rf...
Royal Society of Chemistry
Aspirin—The Wonder of Medicine
What do aspirin and the willow tree have in common? Scholars of chemical synthesis engage in a fascinating reaction to make their own aspirin samples. The lab uses thin layer chromatography analysis, includes stoichiometric calculations,...
Curated OER
Relationships and Biodiversity Lab
In this relationships and biodiversity learning exercise, students investigate the relationships between 4 plants sample using 7 different tests. These include looking at structural characteristics of the plants, seeds and stems, using...
Curated OER
Standard One: Lab Design and Appendix A Lab Skills
In this lab design and lab skills worksheet, students answer questions about experimental design including finding variables, determining controls, and graphing data. They answer questions about microscopy and label the parts of a...
Curated OER
Straw Chromatography
Students become acquainted with the principles of liquid chromatography and construct a liquid chromatography column. Students also investigate the effects of different solvents and solvent concentrations in the separation process.
Curated OER
Using Thin Layer Chromatography To Identify amino Acids In Solution
Learners will work in groups to design and conduct an experiment to identify amino acids found in an unknown solution. This lesson is taught in three sections: (1) Introduction to chromatography (2) Experimental design session (3)...
Curated OER
Paper Chromatography K-12 Experiment
Students study chromatography and how they can distinguish between the different pigments in a color. In this chromatography instructional activity students separate a mixture of dyes in soluble ink then record the colors they see.
Royal Society of Chemistry
Chemistry Masterclass—Chemistry Outreach
Immerse your chemistry class in the world of organic chemistry! Science scholars isolate acetaminophen from an over-the-counter sample during an intense and interesting lab. Groups use many different separation and analysis techniques to...
Curated OER
Using Plant Pigments to Link a Suspect to a Crime
Young scholars use chromatography to separate plant pigments collected from a fictitious crime scene and suspects. They compare the Rf values of the plant pigments to determine whether the plant pigments found on any of the suspects...
Chymist
The Extraction and Identification of Artificial Food Colors
Did you know some food dyes are considered safe while others are considered carcinogenic? During the experiment, young chemists extract artificial food colors from prepared foods. Then, they use chromatography paper to identify which...
Curated OER
Chromatography
Learners conduct various experiments on chromatography. In this chemistry lesson, students distinguish among the different types of chromatography methods. They explain how the stationary and mobile phase interact with each other.
Curated OER
Thin Layer Chromatography (TLC)
Learners study flavinoids and how they exist in the plant kingdom. In this pigment lesson students complete a lab activity to see how flavinoids move in substances then record their observations.
Curated OER
Column Chromatography
Students research the mechanics of liquid chromatography. In this technology instructional activity, students calculate the concentration of unknown samples. They write a report about their research and lab results.
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