Curated OER
Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
Curated OER
A Little More Advanced Biotechnology Tools: Better Plasmids
A nice set of information that will help your students understand the process of using recombinant plasmids. DNA hybridization and blotting mechanisms help illustrate expressed genes and the concepts behind the Human Genome Library....
Curated OER
DNA Technologies
After giving some of the basic characteristics of the DNA molecule, the slides provide process information and many detailed diagrams about plasmids, vectors, and their application in cloning genes.
Curated OER
More Basic Biotechnology Tools, Sorting & Copying DNA
You can use these slides to explain the many complicated steps of the Polymerase chain reaction. The diagnostic and forensic uses of gel electrophoresis are detailed. This PowerPoint could be used to communicate facts, and also to prompt...
Curated OER
Producing a Strain of E. coli that Glows in the Dark
Students observe the experimental process called bacterial transformation and demonstrate phenotype changes in bacteria that have been transformed with an antibiotic-resistance gene and a metabolic marker. They create a luminescent...
Curated OER
A Little More Advanced Biotechnology Tools; Better Plasmids
After using this DNA and plasmids slide show, the steps of the delicate procedures of southern blotting and DNA libraries should be more clear to your students. Students will read the guidelines and are also prompted to answer questions...
Curated OER
DNA Basics, Bacterial Cell Walls
Some basic descriptions of DNA and the appearance of structures in a bacteria are included along with great diagrams. The presentation has clear, but detailed slides, and your students will find the information about Gram staining easy...
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Biotechnology
The concepts here cover the gamut of biotechnological engineering. Information is given about plasmids and genetic manipulation. After reviewing genetic engineering, this slide show would be useful to recap some of the main procedures.
Curated OER
GenEng
Students investigate the process of genetic engineering. For this genetic engineering lesson plan, students use paper models of DNA and plasmid bacterium to investigate the process of replicating DNA, splicing genes from DNA, and...
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Restriction Digest + DNA Comparison
Students compare different DNA and run a gel electrophoresis. In this DNA lesson students complete a DNA activity that allows them to better understand its structure and function.
Cold Spring Harbor Laboratory
Genes Can Be Moved Between Species
Biotechnology changes lives every day, but how did it all start? Learn about Cohen and Boyer's technique for recombinant DNA and the founding of this new scientific study. Then, scholars use the online interactive to discover how Hanahan...
Curated OER
How is Foreign DNA Inserted into Organisms During the Genetic Engineering of Crops?
Students examine the positive benefits and negative consequences of genetically modified organisms following a lecture covering key biotech concepts and techniques. Students then conduct and experiment comparing the travel rate of...
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The Cloning of Plasmid and Spinach DNA
Young scholars will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach...
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Recombinant DNA - Electrophoresis Demonstration and Problem
Suitable for an AP Biology class, the lab found here has teen bioengineers working with plasmids to clone DNA. If you have not done a similar lab, the instructions may be rather difficult to follow. Both the teacher and learner...
Curated OER
Phenotypic and Genotypic Changes in Bacteria
Students investigate a rapid method to render the bacterium Escherichia coli "competent" to uptake plasmid DNA. They demonstrate the phenotypic changes associated with transformation. They perform a cell resuspension from the...
Curated OER
Gene Cloning Worksheet #1
In this gene cloning activity, students are given a gene sequence of DNA, plasmid DNA, 3 restriction enzymes, their cut patterns and a DNA ligase. They answer 6 questions about the results of using different restriction enzymes to cut...
Curated OER
DNA Transformation of Bacteria-Ampicillin
Students demonstrate how genes can be transferred from one bacteria to another. In this experiment, they take a plasmid with a gene for resistance to the antibiotic ampicillin which is used to transfer the resistance gene into a...
Curated OER
Genetic Technology
For this genetic worksheet, students will match 6 terms associated with gene engineering to their correct definition. Then students will complete a table indicating if the vector is mechanical or biological. Finally, students will...
Curated OER
Biotechnology
The intricate details of DNA structure and the double helix are covered in this presentation. Many details about chemical behavior are also explained which will help your students review DNA. Many of the slides are summaries and the 37...
Curated OER
Cloning of Plasmid and Spinach DNA
Young scholars ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and...
Curated OER
A Model of a Bacterial Plasmid
Learners construct a plsmid model with the materials provided and be able to use it to define certain terms and explain other practical applications of genetic engineering. This simulation allows the students to work through the diagram...
Curated OER
Genetic Engineering/Biotechnology
Pupils create a paper model of a plasmid and insert a DNA molecule that contains a specific gene into the plasmid. They complete an actual transformation involving E. coli bacterium and relate the two activities to one another.
Curated OER
Simulation of Gene Splicing
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
Curated OER
Characterization of the Bioluminescence (Lux+) Gene E. coli
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.