Lesson Plan

Chromatographic Separation of Methylene Blue and Sodium Fluorescein

This Chromatographic Separation of Methylene Blue and Sodium Fluorescein lesson plan also includes:

Students determine the best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography. They conduct an initial TLC separation using ethanol and a different solvent mixture and analyze the data of all trials to determine the best solvent.

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