Peas spoilage hot, peas spoilage cold: examine the bacterial growth on newly defrosted peas versus peas that have been defrosted for 24 hours. Using the session one questions in the "Microbes and Food Spoilage" PDF, learners will make predictions about the amount and location of microbial activity they will find after allowing the agar samples to incubate for two to three days. The session two questions give the kids a chance to draw conclusions and analyze their results. The initial session should last about 45 minutes, with the second session coming three days later, also taking approximately 45 minutes.