Curated OER
Cloning of Plasmid and Spinach DNA
Young scholars ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and...
Curated OER
The Cloning of Plasmid and Spinach DNA
Young scholars will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach...