Curated OER
Using Ion Exchange Chromatography to Separate Proteins
Students explore protein purification by separating a positively charged molecule, lysozyme, from a negatively charged molecule, albumin, at neutral pH using ion exchange chromatography.
Curated OER
Can Chromatography Separate A Pigment?
Students separate a mixture of pigments with ion exchange chromatography and predict whether the ion-exchange matrix is positively or negatively charged based on the type of pigment bonded to the matrix.