Curated OER
Characterization of the Bioluminescence (Lux+) Gene E. coli
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
Curated OER
Cloning And b-Galactosidase
Learners investigate cloning using E coli cultures. Through laboratory exercise, they determine if E coli cells contain b - galactosidase.