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Curated OER
Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
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Determining Cutting Site Locations
Students explore DNA restriction mapping. They cut pieces of paper into lengths representing those produced when specific enzymes are used to cut a strand of DNA.
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Science Lesson: Chocolate Flavored Cherries
Students are able to identify start and stop sequences in DNA. They are able to model using restriction enzyme and ligase to remove sections of DNA and reattach them. Students are introduced to the process of recombinat DNA through the...
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How do Restriction Enzymes Work?
Students discover how restriction enzymes work, while learning the importance of restriction enzymes in studying DNA structure.
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Forensic Files: A DNA Dilemma
Seventh-graders come in to science class to find a file on their desks detailing a crime to be solved! As a demonstration, you simulate the restriction of DNA samples and separate them by electrophoresis. From the gel, learners can...
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Simulation of Gene Splicing
Students use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that genetic...
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DNA Fingerprinting through a Chemistry Lens
Students explore DNA and restriction enzymes using fragments of DNA separated by electrophoresis on an agarose gel. They analyze the results to see "whodunit".
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Innocent or Guilty: A Lab on DNA Gel Electrophoresis
Students use the prelab as an introduction to the importance of DNA fingerprinting- a form of identification that us being accepted by both scientific and leagl experts. They prepare a gel for electrophoresis. DNA fragments, which have...
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Cut-off Genes
Investigate the relationships between different deep-sea organisms by DNA sequencing. A worksheet provides instructions for DNA sequencing and space to work. They simulate gel electrophoresis by cutting out paper "DNA strands."...
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Restriction Digest + DNA Comparison
Students compare different DNA and run a gel electrophoresis. In this DNA instructional activity students complete a DNA activity that allows them to better understand its structure and function.
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DNA Recombination II
Students model recombination in DNA. They color the DNA sample and cut it out. Students use their "restriction enzymes" to cut the DNA into specific fragments. They separate the fragments using the "gel electrotrophoresis." Students...
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Gel Electrophoresis: Sort and See the DNA
Students construct DNA fingerprints. In this genetics lesson, students watch an on-line gel electrophoresis animation, then they work in groups to use a restriction map to create a restriction enzyme and use that to arrange a DNA...
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Determination of Phylogenetic Relationships Using Restriction Analysis
Students applied various mathematical analyses to interpret their data. By using the resulting DNA fragment patterns from several enzymes students established a possible evolutionary relationship among the mice.
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Molecular Explorations
Students explain the processes for separating DNA from tissue and separating complex mixtures. In this exploratory lesson students complete a lab where they extract DNA.
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DNA Fingerprinting for High School Biology
Students work with tools and techniques used in genetic engineering. They dissect a sample into fragments of DNA using restriction enzymes, sometimes called "molecular scissors". They sort these fragments, measure and analyze them according
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Characterization of the Bioluminescence (Lux+) Gene E. coli
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
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Gel Electrophoresis
Students conduct a variety of experiments to explore gel electrophoresis. In this biology lesson, explain how this process separate DNA and RNA. They discuss the practical applications of this method.
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COMPARISON OF FOUR-, SIX-, AND EIGHT-bp CUTS IN CALF THYMUS DNA
Learners use this laboratory exercise, restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. Assuming that the four component nucleotides (A, C, T, G) are distributed randomly within a DNA molecule, then any...
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Comparison of Four-, Six-, And Eight-bp Cuts in Calf Thymus Dna
Pupils investigate the use of restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. They utilize restrictive endonucleases to cut Thymus DNA and compare results by viewing the DNA fragments on Agarose Gel...
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The Cloning of Plasmid and Spinach DNA
Students will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in...
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Cloning of Plasmid and Spinach DNA
Students ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and outcome of...
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Gel Electrophoresis Simulation
Students identify the steps in a gel electrophoresis experiment and describe the role of enzymes. They examine the relationship between fragment size and migration rate in a gel. They view DNA fragments as well.
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Simulation of Gene Splicing
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...
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Genetics and Molecular Biology
Students create DNA models using colored construction papers. In this biology lesson, students identify enzyme restriction sites. They model how DNA plasmids replicate when taken up by a bacterial cell.