Catalyst University
Biotechniques | Principles of Gel Electrophoresis (DNA gels)
As always, the steps of DNA electrophoresis depend on your specific lab and protocol. Here, we look at the general theory of separating DNA on an agarose gel by electrophoresis and what it is used for in the lab. We will also learn about...
Catalyst University
Steroidogenesis | The Biosynthesis of Steroids from Cholesterol
In this video, we examine the conversion of cholesterol to all of the major steroid classes: progestogens, mineralocorticoids, glucocorticoids, androgens, and estrogens.
Catalyst University
The Nuclear Pore Complex: Nuclear Import, Export, & RAN
In this video, we discuss the mechanisms by which proteins are targeted to the nucleus (import) and moved from the nucleus to the cytoplasm (export). Collectively, nucleocytoplasmic transport is regulated by the nuclear pore complex and...
Catalyst University
Primer Design for Cloning ENTIRE Gene Sequence
This video explains how to design primers for amplifying an entire gene of interest from start codon (ATG) to STOP codon. -Forward Primer -Reverse Primer -Ordering Primers using IDT
Catalyst University
Prions | Mechanisms of Parkinson's Disease, Parkin Prion, and L-DOPA Treatment
Parkinson's disease is a neurodegenerative prion disease in which affected individuals (e.g., Michael J Fox [comedian]; Muhammad Ali [Heavyweight champ]) suffer cell death in the substantia nigra and tremors. In this video, I will...
NASA
NASA in Silicon Valley: NASA in Silicon Valley Live - Genius Space Hacks
In this episode streamed on Sept. 13, 2018, we talk about studying life in space. Plus, we share some genius space hacks for doing life science research on Earth and aboard the International Space Station.
Catalyst University
Biotechniques | Principles of Primer Design for Full Gene Amplification
In this video, I will show you how to design primers to amplify the entire gene during a routine PCR.
Catalyst University
Using UNIPROT & GenBank to Locate Gene Sequences [Given a Known Protein]
In this video, I show you: 1) How to search UniProt for a protein of interest given its name or code. AND 2) How to access its XM number and gene sequence from UniProt.
Catalyst University
The Mechanism of Insulin Release by Pancreatic β-cells
This video dissects the mechanism by which the Pancreatic β-cells sense blood glucose levels and secrete a proportional amount of insulin through negative feedback.
Catalyst University
Analyzing Gene Sequence Results with BLAST
In this video, I show you how to... 1) obtain gene sequences from a sequencing company (e.g., GeneWiz) 2) Select which part of the sequence is best for analysis 3) analyze results with BLAST
Catalyst University
Amino Acid Biosynthesis | Biosynthesis of Tryptophan
In this video, I discuss the biosynthesis of the third aromatic amino acid, Tryptophan, from chorismate which occurs in the plastid (chloroplast).
Catalyst University
Biotechniques | Basics of Making His-Tags & Nickel Affinity Chromatography
As always, the steps of putting a gene insert into vector/plasmid AND nickel affinity chromatography depend on your specific lab and protocol. Here, we look at the general theory of engineering a poly-His tagged protein and Ni affinity...
Catalyst University
Prions | Mechanisms of Huntington's Disease & the Huntingtin Prion
Huntington's disease is a neurodegenerative prion disease in which affected individuals suffer destruction of neurons in the basal nuclei (e.g., GABAergic neurons) and rapid, involuntary movements as a result. In this video, I will...
Catalyst University
Vitamins and Minerals | Vitamin C Biosynthesis & the GULO Pseudogene
Here, we explore the biosynthetic pathway for vitamin C (ascorbic acid) from glucuronic acid. Also, we explore inability of some organisms (e.g., humans) to perform this biosynthesis due to the GULO pseudogene.
Catalyst University
Prions | Mechanisms and Theories of Alzheimers Disease (Amyloid & Tau)
Alzheimer's disease is a neurodegenerative prion disease in which affected individuals suffer destruction of cortical neurons due to accumulation of prions of both beta-amyloid and Tau type. In this video, I will explain the acquisition...
Catalyst University
The Biotin Cycle | Mammalian Biotin (Vitamin B7) Metabolism & Processing
This video explores the biotin cycle. How is biotin attached to an enzyme? How is it removed? Is biotin recycled?
Catalyst University
Biological Fate of Artificial Sweeteners | Erythritol & Sorbitol
Did you ever wonder what happens to artificial sweeteners when you consume them in food? In this video, we track the fates of two of them (erythritol and sorbitol). They cause loose stool, but they can have other functions as well.
Catalyst University
Progeria, Accelerated Aging | Biochemical Mechanism of Progeria
In this video, I explain the mechanism of progeria, a rare but fatal accelerated aging disease. We will explore the synthesis pathway for normal wild-type Lamin A and the mutant form, progerin, for which the disease is named.
Brainwaves Video Anthology
Bruce D. Baker - Teachers Make a Difference - Wayne Leibel
Bruce D. Baker, Ed.D. is a Professor in the Department of Educational Theory, Policy, and Administration at the GSE, and is widely recognized as a leading scholar in the field of education finance. Serving in both academic and...
Catalyst University
Lactase & the Mechanism of Lactose Intolerance
1) What is the function of the enzyme, lactase? 2) What is the cause of the flatulence and diarrhea associated with lactose intolerance?
Catalyst University
Drugs & Xenobiotics | Mechanisms of Arsenic Poisoning
Here, we will see how two forms of arsenic, arsenate and arsenote, disrupt energy metabolism via glycolysis and the TCA cycle, respectively.
Catalyst University
Intestinal Sulfate Reduction to H2S by Sulfur-Reducing Bacteria
In this video, we will explore the source of the major cause of the smell of flatulence. Intestinal sulfate-reducing bacteria reduce sulfate to hydrogen sulfide (H2S). We will see the biochemical pathway here and how it is coupled to...
Catalyst University
Exercise Physiology | What is Supramaximal Exercise?
What is Supramaximal Exercise? In this video, I explain the concept of supramaximal exercise using a metabolic approach.
Catalyst University
How to Interpret Ramachandran Plots
In this video, I explain the purpose of Ramachandran Plots and how to interpret them.