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Lesson Plan
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Teach Engineering

Restriction Enzymes and DNA Fingerprinting

For Teachers 9th - 12th
Show your class why restrictions aren't always a bad thing. In the third segment of a four-part series, the instructor develops the idea of restriction enzymes. Pupils learn how scientists use restriction enzymes in DNA analysis and DNA...
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PPT
Curated OER

Biotechnology

For Teachers 9th - 12th
The metaphors used here to describe the "cut and paste" procedures for genetic engineering will really help your biologists in their understanding of restriction enzymes and DNA.  The uses of genes that are isolated and incorporated into...
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Lesson Plan
Curated OER

Using a Controlled Experiment to Identify Two Unknown Plasmids

For Teachers 10th - 12th
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
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Lesson Plan
Curated OER

From Restriction Maps to Cladograms

For Teachers 9th - 12th
Biology aces analyze restriction maps to determine the relationships among different primate species and then complete a cladogram. Preface this lesson with an explanation of how restriction enzymes are used to create the DNA maps....
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Lesson Plan
Curated OER

Determining Cutting Site Locations

For Teachers 7th - 12th
Students explore DNA restriction mapping. They cut pieces of paper into lengths representing those produced when specific enzymes are used to cut a strand of DNA.
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Lesson Plan
Curated OER

Enzyme and Southern Blotting

For Teachers 9th - 12th
Young scholars study southern blotting and its uses. They research on restriction enzymes and their uses, especially in relation to recombinant DNA. In addition, they write a summary and an interpretation of the gel.
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Lesson Plan
Curated OER

Science Lesson: Chocolate Flavored Cherries

For Teachers 9th - 12th
Students are able to identify start and stop sequences in DNA. They are able to model using restriction enzyme and ligase to remove sections of DNA and reattach them. Students are introduced to the process of recombinat DNA through the...
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Worksheet
Curated OER

Gene Cloning Worksheet #1

For Students 9th - 12th
In this gene cloning activity, students are given a gene sequence of DNA, plasmid DNA, 3 restriction enzymes, their cut patterns and a DNA ligase. They answer 6 questions about the results of using different restriction enzymes to cut...
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Lesson Plan
Curated OER

How do Restriction Enzymes Work?

For Teachers 8th - 10th
Students discover how restriction enzymes work, while learning the importance of restriction enzymes in studying DNA structure.
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Worksheet
Curated OER

AP: Chapter 20: DNA Technology

For Students 9th - Higher Ed
This nine-page biotechnology resource contains six pages of questions regarding DNA technology. Questions cover cloning, restriction enzymes, the polymerase chain reaction, different techniques, the human genome project, and more. The...
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PPT
Curated OER

Biotechnology

For Teachers 9th - 12th
The concepts here cover the gamut of biotechnological engineering. Information is given about plasmids and genetic manipulation. After reviewing genetic engineering, this slide show would be useful to recap some of the main procedures.
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Lesson Plan
Curated OER

Forensic Files: A DNA Dilemma

For Teachers 7th - 12th
Seventh-graders come in to science class to find a file on their desks detailing a crime to be solved! As a demonstration, you simulate the restriction of DNA samples and separate them by electrophoresis. From the gel, learners can...
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Lesson Plan
Curated OER

Restriction Enzymes

For Teachers 10th - 12th
Students explore enzymes and enzyme combinations. They examine and cut up and practice matching DNA sequences.
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Worksheet
Curated OER

Who Ate The Cheese?!

For Students 10th - 12th
In this crime scene investigation worksheet, students simulate DNA sequencing and DNA restriction analysis using paper strips of DNA. Students compare the crime scene DNA to the suspects and determine who is guilty.
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Lesson Plan
Curated OER

Simulation of Gene Splicing

For Teachers 9th - 12th
Students use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that genetic...
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Lesson Plan
Curated OER

DNA Fingerprinting through a Chemistry Lens

For Teachers 6th - 12th
Young scholars explore DNA and restriction enzymes using fragments of DNA separated by electrophoresis on an agarose gel. They analyze the results to see "whodunit".
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Lesson Plan
Curated OER

Innocent or Guilty: A Lab on DNA Gel Electrophoresis

For Teachers 8th - 12th
Young scholars use the prelab as an introduction to the importance of DNA fingerprinting- a form of identification that us being accepted by both scientific and leagl experts. They prepare a gel for electrophoresis. DNA fragments, which...
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Worksheet
Curated OER

Genetic Technology

For Students 9th - Higher Ed
In this genetic worksheet, students will match 6 terms associated with gene engineering to their correct definition. Then students will complete a table indicating if the vector is mechanical or biological. Finally, students will...
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Lesson Plan
Curated OER

DNA Fingerprinting with Restriction Enzymes

For Teachers 6th - 12th
Young scholars will illustrate how restriction enzymes work by piecing together parts of a puzzle. Then they apply this knowledge to help them analyze genetic evidence from the scene of a crime as well as to help them comprehend how a...
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Lesson Plan
Curated OER

Cut-off Genes

For Teachers 9th - 12th
Investigate the relationships between different deep-sea organisms by DNA sequencing. A worksheet provides instructions for DNA sequencing and space to work. They simulate gel electrophoresis by cutting out paper "DNA strands." Modeling...
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Lesson Plan
Curated OER

The Clark Family Story: Tracing the Cause of Hemophilia

For Teachers 7th - 12th
Ms. Strohfeldt did not omit anything when she designed this comprehensive lesson plan on DNA mutation and sex-linked traits. Begin with a pretest as an anticipatory set. Read a case history of the Clark family and the occurrence of...
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Worksheet
Curated OER

Fly Forensics

For Students 7th - 12th
Freddy's Friendly Fruit Market is under attack by fruit flies, and it is up to your aspiring biologists to solve the crime! By reading and analyzing the phenotypes, detectives determine which fly is responsible. This memorable assignment...
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Lesson Plan
Curated OER

Restriction Digest + DNA Comparison

For Teachers 9th - 12th
Students compare different DNA and run a gel electrophoresis.  In this DNA lesson students complete a DNA activity that allows them to better understand its structure and function. 
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Lesson Plan
Curated OER

lambda DNA Fingerprinting Simulation

For Teachers 11th - 12th
Students perform restriction digests on DNA samples from four individuals, and then search for similarities between the individuals by running the restriction fragments on an electrophoresis gel.

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