Teach Engineering
DNA Forensics and Color Pigments
Use food coloring in electrophoresis. The last segment in a four-part series mimics DNA fingerprinting by using chromatography. Teams conduct chromatography on food coloring to find colors that use similar pigmentation in their makeup.
Curated OER
DNA Recombination II
Students model recombination in DNA. They color the DNA sample and cut it out. Students use their "restriction enzymes" to cut the DNA into specific fragments. They separate the fragments using the "gel electrotrophoresis." Students...
Curated OER
Gel Electrophoresis: Sort and See the DNA
Students construct DNA fingerprints. In this genetics lesson, students watch an on-line gel electrophoresis animation, then they work in groups to use a restriction map to create a restriction enzyme and use that to arrange a DNA...
Teach Engineering
Imagining DNA Structure
Let's get a closer look at DNA and other molecular structures. The first lesson in the series of four introduces a variety of imagining techniques that engineers and scientists use to visualize molecular structures. The resource presents...
Curated OER
cDNA Sequencing By Restriction Project
Students try to confirm the sequence of a given cDNA clone using the WWW tools and traditional restriction analysis. They grow the clone, prep the plasmid, cut the plasmid with restriction enzymes and compare their results to what was...
Curated OER
Determination of Phylogenetic Relationships Using Restriction Analysis
Students applied various mathematical analyses to interpret their data. By using the resulting DNA fragment patterns from several enzymes students established a possible evolutionary relationship among the mice.
Curated OER
Molecular Explorations
Students explain the processes for separating DNA from tissue and separating complex mixtures. In this exploratory lesson students complete a lab where they extract DNA.
Curated OER
Gel Electrophoresis: Sort and See the DNA
In this DNA learning exercise, learners access a website to learn how DNA fragments are separated through the process of gel electrophoresis. Then students model the construction of DNA fingerprints and practice interpreting restriction...
Curated OER
Science: Designer genes
Students engineer new organisms using biotechnology. In small groups, they write procedures, list benefits and drawbacks, and explain how their new organism might affect the environment. After creating their new organisms, they present...
Curated OER
DNA Race Track
Twelfth graders simulate a DNA fragment running through a gel electrophoresis in a class activity. Students work in teams as a DNA sample with each person on the team representing a fragment that moves through the gel at a specific speed.
Curated OER
DNA Fingerprinting for High School Biology
Students work with tools and techniques used in genetic engineering. They dissect a sample into fragments of DNA using restriction enzymes, sometimes called "molecular scissors". They sort these fragments, measure and analyze them according
Curated OER
Characterization of the Bioluminescence (Lux+) Gene E. coli
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
Curated OER
Gel Electrophoresis
Young scholars conduct a variety of experiments to explore gel electrophoresis. In this biology lesson, explain how this process separate DNA and RNA. They discuss the practical applications of this method.
Towson University
Case of the Crown Jewels
Can your biology class crack the Case of the Crown Jewels? Junior forensics experts try their hands at DNA restriction analysis in an exciting lab activity. The activity introduces the concept of restriction analysis, teaches pipetting...
Curated OER
COMPARISON OF FOUR-, SIX-, AND EIGHT-bp CUTS IN CALF THYMUS DNA
Students use this laboratory exercise, restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. Assuming that the four component nucleotides (A, C, T, G) are distributed randomly within a DNA molecule, then any...
Cold Spring Harbor Laboratory
The RNA Message Is Sometimes Edited
In 1993, Phillip Sharp and Richard Roberts won the Nobel Prize for their discovery of split gene theory. Learn about the breakthrough with the help of an online interactive. Hear both scientists explain it in their own words, watch an...
Curated OER
DNA Technology
In this DNA technology worksheet, students will review 12 vocabulary words relating to the uses and results of DNA technology in this crossword puzzle.
Curated OER
Comparison of Four-, Six-, And Eight-bp Cuts in Calf Thymus Dna
Students investigate the use of restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. They utilize restrictive endonucleases to cut Thymus DNA and compare results by viewing the DNA fragments on Agarose Gel...
Curated OER
The Cloning of Plasmid and Spinach DNA
Young scholars will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach...
Curated OER
DNA Fingerprinting
Ninth graders investigate what restriction enzymes do, and explain how a DNA sample can link a suspect to a crime.
Curated OER
Cloning of Plasmid and Spinach DNA
Young scholars ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and...
Curated OER
From Restriction Maps to Cladograms
Students develop a matrix to determine the number of differences between each set of primate species. They determine closely related organisms then complete the cladogram and answer a set of questions.
Curated OER
Outbreak! Electrophoresis: Where the Bands Are
Students examine why pieces of DNA move at different rates and over different distances. In this DNA lesson students complete an activity.
Curated OER
Gel Electrophoresis Simulation
Students identify the steps in a gel electrophoresis experiment and describe the role of enzymes. They examine the relationship between fragment size and migration rate in a gel. They view DNA fragments as well.
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