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\nPyrosequencing\n

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\nPyrosequencing is a DNA sequencing-by-synthesis method that detects light emitted during nucleotide incorporation, offering a faster and more cost-effective alternative to the traditional Sanger method. It uses a cascade of enzymatic...\nPyrosequencing is a DNA sequencing-by-synthesis method that detects light emitted during nucleotide incorporation, offering a faster and more cost-effective alternative to the traditional Sanger method. It uses a cascade of enzymatic reactions involving DNA polymerase, ATP sulfurylase, luciferase, and apyrase to generate light signals that correspond to nucleotide addition. Although limited to sequencing shorter DNA fragments (300–500 bp), it is highly accurate, automatable, and ideal for applications like SNP detection and short-read analysis.\n
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Resource Details

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Curator Rating
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Educator Rating
Not yet Rated
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Media Length
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7:19
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Grade
9th - Higher Ed
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Subjects
Science
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Media Type
Instructional Videos
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Source:
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\nBioMagica\n\n
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Date
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2019
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Language
English
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Audiences
For Teacher Use
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Usage Permissions
Fine Print: Educational Use
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Concepts

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hydrogen bonds, light, adenine, addition, cytosine, thymine, nucleotides, guanine
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Additional Tags

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csir, net, pyrosequencing, luciferase, atp, atp sulfurylase, pyrophosphate, dna sequencing, analysis of dna sequence from pyrosequencing graph, pyro sequencer, daughter strand, dna polymerase, enzyme apyrase, enzyme luciferase, thymine molecules, light signal, peak, signal, pyramidines, generation, sequence, luciferin, nucleotide, purines, thiamine, provide, accepted, cycle, provided
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