{"page":"\u003clink rel=\"stylesheet\" href=\"https://lessonplanet.com/assets/packs/css/resources-c03aa079.css\" /\u003e\n\u003clink rel=\"stylesheet\" href=\"https://lessonplanet.com/assets/packs/css/lp_boclips_stylesheets-517835be.css\" media=\"all\" /\u003e\n\u003cdiv data-title='Fate of the Northern White Rhino now in the hands of scientists' data-url='/boclips/videos/5c54d758d8eafeecae20b1be' data-video-url='/boclips/videos/5c54d758d8eafeecae20b1be' id='bo_player_modal'\u003e\n\u003cdiv class='boclips-resource-page modal-dialog panel-container'\u003e\n\u003cdiv class='react-notifications-root'\u003e\u003c/div\u003e\n\u003cdiv class='rp-header'\u003e\n\u003cdiv class='rp-type'\u003e\n\u003ci aria-hidden='true' class='fai fa-regular fa-circle-play'\u003e\u003c/i\u003e\nVideo\n\u003c/div\u003e\n\u003ch1 class='rp-title' id='video-title'\u003e\nFate of the Northern White Rhino now in the hands of scientists\n\u003c/h1\u003e\n\u003cdiv class='rp-actions'\u003e\n\u003cdiv class='mr-1'\u003e\n\u003ca class=\"btn btn-success\" data-posthog-event=\"Signup: LP Signup Activity\" data-posthog-location=\"body_link_boclips\" data-remote=\"true\" href=\"/subscription/new\"\u003e\u003cspan\u003e\u003cspan\u003eGet Free Access\u003c/span\u003e\u003cspan class=\"\"\u003e for 10 Days\u003c/span\u003e\u003cspan\u003e!\u003c/span\u003e\u003c/span\u003e\u003c/a\u003e\n\u003c/div\u003e\n\u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv class='rp-body'\u003e\n\u003cdiv class='rp-info'\u003e\n\u003cdiv aria-label='Hide resource details' class='rp-hide-info' role='button' tabindex='0'\u003e\u0026times;\u003c/div\u003e\n\u003ci aria-label='Expand resource details' class='rp-expand-info fai fa-solid fa-up-right-and-down-left-from-center' role='button' tabindex='0'\u003e\u003c/i\u003e\n\u003ci aria-label='Compress resource details' class='rp-compress-info fai fa-solid fa-down-left-and-up-right-to-center' role='button' tabindex='0'\u003e\u003c/i\u003e\n\u003cdiv class='rp-rating'\u003e\n\u003cspan class='resource-pool'\u003e\n\u003cspan class='pool-label'\u003ePublisher:\u003c/span\u003e\n\u003cspan class='pool-name'\u003e\n\u003cspan class='text'\u003e\u003ca data-publisher-id=\"30356011\" href=\"/search?publisher_ids%5B%5D=30356011\"\u003eCurated Video\u003c/a\u003e\u003c/span\u003e\n\u003c/span\u003e\n\u003c/span\u003e\n\u003c/div\u003e\n\u003cdiv class='rp-description'\u003e\n\u003cspan class='short-description'\u003eLEADIN:              Scientists say they are confident that efforts to reproduce the northern white rhino using IVF will be successful.               The world's last male northern white rhino died on Monday, leaving just two ageing...\u003c/span\u003e\n\u003cspan class='full-description hide'\u003eLEADIN:\u003cbr/\u003e              Scientists say they are confident that efforts to reproduce the northern white rhino using IVF will be successful. \u003cbr/\u003e              The world's last male northern white rhino died on Monday, leaving just two ageing females of the subspecies on the planet.\u003cbr/\u003e              Now conservationists across globe are redoubling their efforts to use frozen sperm and a surrogate female to save the Northern White from extinction.  \u003cbr/\u003e              STORYLINE\u003cbr/\u003e              In the end, the keepers at the Ol Pejeta Conservancy in Kenya  were left with little other choice than to euthanise the world's last remaining northern white rhino. \u003cbr/\u003e              Sudan - who was 45 years old -  passed away Monday (19th March 2018) after an infection had left him unable to walk and in constant pain. \u003cbr/\u003e              The rhino had been part of an ambitious effort to save the subspecies from extinction with the help of the two surviving females. One is his daughter, Najin, and the other is his granddaughter, Fatu. \u003cbr/\u003e              But Sudan's death does not mark the end of his contribution to save his kind. His genetic material has been collected and added to the stock of white rhino sperm that remains the only hope for sub-species.  \u003cbr/\u003e              The Leibniz Institute for Zoo and Wildlife Research (IZW) in Berlin is part of group of specialists from around the world that is striving to save the sub-species using IVF techniques with stored semen from other dead rhinos and eggs extracted from the two remaining females.\u003cbr/\u003e              Collaborators include, San Diego Zoo Global in the U.S.,  ZOO Dvur Kralove of the Czech Republic, Ol Pejeta Conservancy in Kenya, Tokyo University, Scripps Research Institute and other international and domestic entities. \u003cbr/\u003e              \"This is a really alarming development, and particularly with regard to 'Sudan' it is absolutely severe and very sad. The question is what can we still do now? This is one of the main questions, and for us as scientists at the Leibniz Institute for Zoo and Wildlife Research it means that we have the possibility of making a significant contribution with our international team. We have two options: one option is stem cell research which is still very futuristic but we are working on it, and the currently more interesting and more realistic option is in-vitro fertilisation,\" says IZW spokesman Steven Seet.\u003cbr/\u003e              Seet hopes to be able to transfer an embryo to a surrogate in the next two years. A successful pregnancy would then involve an 18 month wait for the birth. \u003cbr/\u003e              \"This (in-vitro fertilisation) is about taking ovules from the two remaining cows in Kenya, and then mixing these ovules them with sperm of northern white rhinos that we are storing here in our conservation cooling, then growing an embryo and freezing this embryo for the time being, to win time so that we can accomplish the embryo transfer to a surrogate mother, a southern white rhino cow. This really is the most likely option,\" he says. \u003cbr/\u003e              In the U.S. Dr. Barbara Durrant, Director of Reproductive Sciences at the San Diego Zoo Institute for Conservation Research and San Diego Zoo Global is also hoping to use frozen sperm and eggs from deceased northern white rhinos, and someday through artificial insemination, in vitro fertilisation and embryo transfer, use a southern white rhinos as a surrogate mother.\u003cbr/\u003e              \"So the southern white rhinos that we saw over at the Nikita Kahn Rhino Rescue Center are here for research purposes but also to contribute to the north American population. We will be developing artificial insemination techniques and embryo transfer techniques with these six females, so that they will be proven females that we will know that they have proven themselves capable of carrying a fetus to term before we would risk putting a precious northern white rhino embryo into one of those southern white rhinos as a surrogate. So we'll be developing the techniques to grow embryos, to mature eggs, to fertilize eggs, and grow those embryos to the blastocyst stage and then develop the techniques for embryo transfer. This is a vial Angalifu's sperm that was collected post-mortem and we have only a hundred vials of his sperm to last us forever so we'll be very, very careful with it.\"says Dr. Durrant.\u003cbr/\u003e              Researchers are also looking at using 12 northern white rhino cell lines from San Diego's Frozen Zoo to develop stem cells to create northern white rhino sperm and oocytes for the generation of embryos. \u003cbr/\u003e              \"We will do a technique called ICSI, Intracytoplasmic Sperm Injection, where we can file off this file and take a single sperm and inject it into a single egg so we can save the sperm for genereations to come, and this is a technique that's used widely in humans, and cattle, and horses and we are developing it and we've already had some success in the rhino,\" says Dr. Durrant. \u003cbr/\u003e              Success may be years away, but Dr Durrant is confident that a live birth will happen eventually. \u003cbr/\u003e              \"In these tanks we have sperm and testicular tissue and cell lines from 12 northern white rhinos. So we have enough genetic diversity - all of those cell lines been sequenced, their genome's been sequenced  -  and we know that we have enough genetic diversity in those 12 cell lines to reproduce a population of northern white rhinos.\"\u003cbr/\u003e              There's little choice than to do otherwise.  The fate of the northern white rhino now lies in the laboratory. \u003cbr/\u003e              Northern white rhinos once roamed parts of Chad, Sudan, Uganda, Congo and Central African Republic, and were particularly vulnerable because of the armed conflicts that have swept the region over decades.\u003cbr/\u003e            \u003cbr/\u003e\u003cbr/\u003e              Ol Pejeta Conservancy, Nanyuki, Kenya - 10 February 2016\u003cbr/\u003e              1. Wide of Sudan, the last male Northern White Rhino \u003cbr/\u003e              \u003cbr/\u003e              Berlin, Germany; 20 March 2018\u003cbr/\u003e              2. Skull of rhino in glass cabinet\u003cbr/\u003e              3. Skull of white rhino in glass cabinet\u003cbr/\u003e              4. Steven Seet of the Leibniz Institute for Zoo and Wildlife Research (IZW) talking behind glass cabinet\u003cbr/\u003e              5. SOUNDBITE (German) Steven Seet, spokesman, Leibniz Institute for Zoo and Wildlife Research (IZW):\u003cbr/\u003e              \"This is a really alarming development, and particularly with regard to 'Sudan' it is absolutely severe and very sad. The question is what can we still do now? This is one of the main questions, and for us as scientists at the Leibniz Institute for Zoo and Wildlife Research it means that we have the possibility of making a significant contribution with our international team. We have two options: one option is stem cell research which is still very futuristic but we are working on it, and the currently more interesting and more realistic option is in-vitro fertilisation.\"\u003cbr/\u003e              \u003cbr/\u003e              Nanyuki, Central Kenya - 2 March 2018 \u003cbr/\u003e              6. Various of female white rhinos - the Northern whites are the cows that are lying down \u003cbr/\u003e              \u003cbr/\u003e              Berlin, Germany; 20 March 2018\u003cbr/\u003e              7. SOUNDBITE (German) Steven Seet, spokesman, Leibniz Institute for Zoo and Wildlife Research (IZW):\u003cbr/\u003e              \"This (in-vitro fertilisation) is about taking ovules from the two remaining cows in Kenya, and then mixing these ovules with the sperm of northern white rhinos that we are storing here in our conservation cooling, then growing an embryo and freezing this embryo for the time being, to win time so that we can accomplish the embryo transfer to a surrogate mother, a southern white rhino cow. This really is the most likely option.\"\u003cbr/\u003e              8. Scientist Dr. Robert Hermes opening conservation cooling container storing cell cultures and sperm\u003cbr/\u003e              9. Samples of cell cultures being taken out of container\u003cbr/\u003e              10. Dr. Hermes closing container\u003cbr/\u003e              \u003cbr/\u003e              San Diego Zoo Safari Park, Escondido, California - March 19, 2018\u003cbr/\u003e              11. Wide shot of female Southern White Rhinoceros, Amani\u003cbr/\u003e              12. Wide shot of Amani walking into holding pen for ultrasound.\u003cbr/\u003e              13. Wide shot of Amani being fed by zookeeper as Dr. Barbara Durrant, Director of reproductive sciences at the San Diego Zoo Institute for Conservation Research, and researcher, Parker Pennington, prepare for ultrasound.\u003cbr/\u003e              14. Tight shot of zookeeper feeding Amani to calm her before ultrasound.\u003cbr/\u003e              15. Tight shot of zookeeper feeding Amani.\u003cbr/\u003e              16. Wide shot of Dr. Barbara Durrant and Parker Pennington, starting ultrasound.\u003cbr/\u003e              17. Medium shot of researcher Parker Pennington administering ultrasound. \u003cbr/\u003e              18. Medium shot of Dr. Durrant and Pennington looking at ultrasound.\u003cbr/\u003e              19. Tight shot of Pennington with arm in Amani while administering ultrasound. \u003cbr/\u003e              20. Tight shot of ultrasound of Amani who has been artificially inseminated. \u003cbr/\u003e              21. Wide ultrasound\u003cbr/\u003e              22. Close ultrasound\u003cbr/\u003e              23. SOUNDBITE (English)  Parker Pennington, Researcher, San Diego Zoo Institute for Conservation Research: \u003cbr/\u003e              \"I've done horses and cattle before but this is a whole new kind of learning experience for me. They're, they're obviously quite a bit bigger than horses and cattle but um, the principles are the same, they're just a bit heavier, it's bit heavier in there and when they squeeze, it's quite the squeeze.\"\u003cbr/\u003e              24. Wide shot of frozen zoo and Dr. Durrant\u003cbr/\u003e              25. Medium shot of Dr Durrant filling trays with nitrogen\u003cbr/\u003e              26. SOUNDBITE (English) Dr. Durrant, Director of reproductive sciences at the San Diego Zoo Institute for Conservation Research: \u003cbr/\u003e              \"We do have Angalifu's sperm in here. We have a number of other things in here, um, I\"ll just pull a couple of them out. Um this is Jao's box turtle, this is one of the most endangered reptiles on the planet, and we collected this sample post-mortem.\"\u003cbr/\u003e              27. Tight shot of tubes \u003cbr/\u003e              28. SOUNDBITE (English)  Dr. Durrant, Director of reproductive sciences at the San Diego Zoo Institute for Conservation Research: \u003cbr/\u003e              \"So the southern white rhinos that we saw over at the Nikita Kahn Rhino Rescue Center are here for research purposes but also to contribute to the north American population. We will be developing artificial insemination techniques and embryo transfer techniques with these six females, so that they will be proven females that we will know that they have proven themselves capable of carrying a fetus to term before we would risk putting a precious northern white rhino embryo into one of those southern white rhinos as a surrogate. So we'll be developing the techniques to grow embryos, to mature eggs, to fertilize eggs, and grow those embryos to the blastocyst stage and then develop the techniques for embryo transfer. This is a vial Angalifu's sperm that was collected post-mortem and we have only a hundred vials of his sperm to last us forever so we'll be very, very careful with it.\"\u003cbr/\u003e              29. Tight shot of vial container\u003cbr/\u003e              30. Medium shot of vials of frozen sperm\u003cbr/\u003e              31. SOUNDBITE (English) Dr. Durrant, Director of reproductive sciences at the San Diego Zoo Institute for Conservation Research: \u003cbr/\u003e              \"We will do a technique called ICSI, Intracytoplasmic Sperm Injection, where we can file this file and take a single sperm and inject it into a single egg so we can save the sperm for genereations to come, and this is a technique that's used widely in humans, and cattle, and horses and we are developing it and we've already had some success in the rhino.\"\u003cbr/\u003e              32. Wide shot of Dr. Durrant putting vials back in frozen tank.\u003cbr/\u003e              33.  SOUNDBITE (English) Dr. Durrant, Director of reproductive sciences at the San Diego Zoo Institute for Conservation Research: \u003cbr/\u003e              \"In these tanks we have sperm and testicular tissue and cell lines from 12 northern white rhinos. So we have enough genetic diversity - all of those cell lines been sequenced, their genomes have been sequenced  -  and we know that we have enough genetic diversity in those 12 cell lines to reproduce a population of northern white rhinos.\"\u003cbr/\u003e              \u003cbr/\u003e              Ol Pejeta Conservancy, Nanyuki, Kenya - 10 February 2016\u003cbr/\u003e              34. 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